tsRNA-15797-modified BMSC-derived exosomes mediate LFNG to induce angiogenesis in osteonecrosis of the femoral head

Background: Osteonecrosis of the femoral head (ONFH) is an ischemic disease characterized by impairment angiogenesis. We have previously elucidated role tsRNAs and BMSC exosomes in ONFH, but whether tsRNA-modified promote angiogenesis ONFH remains unclear. Methods: The expression angiogenesis-related tsRNA plasma from patients was examined q-PCR. function HUVECs identified CCK-8 assay. Exosomes purified tsRNA-15797 overexpressed BMSCs were cocultured with to examine their molecule mechanism tsRNA-15797-modified explored RNA sequencing, dual-luciferase assay, immunofluorescence. Results: A tRNA-derived small down-regulated patients. found effects BMSCs-derived on accelerating migration, which further enhanced after overexpressing tsRNA-15797. Besides, overexpression would lead down-regulation LFNG correlated could directly interact LFNG. demonstrated that LNFG weakened pro angiogenic migratory exosomes. Conclusion: successfully constructed BMSC-derived it induced targeting Thus, tsRNA-15797-loaded may be a potential target therapy drug for ONFH.